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Cas9とHistone variantとの融合によるNHEJ抑制をPLOS ONEで発表/NHEJ suppression by fusion of Cas9 and Histone variants published in PLOS ONE


文責:加藤-乾 朋子

CRISPR-Cas9 and other genome editing technologies are expected to be applied in various fields including medicine. However, they mainly induce base deletions and insertions by non-homologous end joining (NHEJ), and the infrequency of editing by homology-directed repair (HDR) is a challenge. In this study, we found that fusing a histone variant (variant) to Cas9 suppresses NHEJ activity. H2A.X, a variant of histone, is known to be involved in DNA repair, suppressed NHEJ activity when fused to Cas9, but H2A.1 was found to be the most effective in suppressing NHEJ. Histones contribute to the formation of stable nucleosome complexes in cells by being wrapped around by DNA, but the suppression of NHEJ activity by fusion with Cas9 was unexpected. Although further studies are needed to elucidate the detailed molecular mechanism, Cas9 fused with histone variants is a useful tool for suppressing NHEJ.

Written by Tomoko Kato-Inui


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