2018

Detection and Quantification of HDR and NHEJ Induced by Genome Editing at Endogenous Gene Loci Using Droplet Digital PCR.

Miyaoka Y, Mayerl SJ, Chan AH, Conklin BR#.

Methods Mol Biol. 2018;1768:349-362.

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair.

Kato-Inui K, Takahashi G, Hsu S, Miyaoka Y#.

Nucleic Acids Research. 2018 May 18;46(9):4677-4688.

2017

Engineered human pluripotent stem cell-derived intestinal tissues with a functional enteric nervous system.

Workman MJ*, Mahe MM*, Trisno S, Poling HM, Watson CL, Sundaram N, Chang CF, Schiesser J, Aubert P, Stanley EG, Elefanty AG, Miyaoka Y, Mandegar MA, Conklin BR, Neunlist M, Brugmann SA, Helmrath MA, Wells JM#

Nature Medicine. 2017 Jan;23(1):49-59. 

2016

Ligand-binding domains of nuclear receptors facilitate tight control of split CRISPR activity​.

Nguyen DP, Miyaoka Y, Gilbert LA, Mayerl SJ, Lee BH, Weissman JS, Conklin BR, Wells JA#.

Nature Communications. 2016 Jul 1;7:12009.

Using Digital Polymerase Chain Reaction to Detect Single-Nucleotide Substitutions Induced by Genome Editing.

Miyaoka Y, Chan AH, Conklin BR#.

Cold Spring Harbor Protocols. 2016 Jun 1.

Detecting Single-Nucleotide Substitutions Induced by Genome Editing. (Introduction for the protocol above)

Miyaoka Y, Chan AH, Conklin BR#.

Cold Spring Harbor Protocols. 2016 Jun 1.

Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing.

Miyaoka Y*#, Berman JR*, Cooper SB, Mayerl SJ, Chan AH, Zhang B, Karlin-Neumann GA, Conklin BR#.

Scientific Reports. 2016 Mar 31;6:23549.

 

CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs.

Mandegar MA#, Huebsch N, Frolov EB, Shin E, Truong A, Olvera MP, Chan AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP, Weissman JS, Qi LS, So PL, Conklin BR#.

Cell Stem Cell. 2016 Apr 7;18(4):541-553.

 

-2015

Detection of Genome Editing Events by Digital PCR.

Miyaoka Y.

TheScientist Webinar Series. Advancing Genomic Medicine: Exploring Applications of Digital PCR, 2015 Oct 14.

(Web seminar)

 

Oncostatin M maintains the hematopoietic microenvironment in the bone marrow by modulating adipogenesis and osteogenesis.

Sato F, Miyaoka Y, Miyajima A, Tanaka M#.

PLoS One. 2014 Dec 31;9(12):e116209.

 

Isolation of single-base genome-edited human iPS cells without antibiotic selection. (PMC Ver.)

Miyaoka Y, Chan AH, Judge LM, Yoo J, Huang M, Nguyen TD, Lizarraga PP, So PL, Conklin BR#.

Nat Methods. 2014 Mar;11(3):291-3. (Please request full-text via email)

 

To divide or not to divide: revisiting liver regeneration.

Miyaoka Y, Miyajima A#.

Cell Div. 2013 Jun 20;8(1):8.

Recommended in F1000.

 

Hypertrophy and unconventional cell division of hepatocytes underlie liver regeneration.

Miyaoka Y, Ebato K, Kato H, Arakawa S, Shimizu S, Miyajima A#.

Curr Biol. 2012 Jul 10;22(13):1166-75.

Recommended in F1000.

 

Hepatic stellate cell-derived delta-like homolog 1 (DLK1) protein in liver regeneration.

Zhu NL, Asahina K, Wang J, Ueno A, Lazaro R, Miyaoka Y, Miyajima A, Tsukamoto H#.

J Biol Chem. 2012 Mar 23;287(13):10355-67.

 

Retention in the Golgi apparatus and expression on the cell surface of Cfr/Esl-1/Glg-1/MG-160 are regulated by two distinct mechanisms.

Miyaoka Y*, Kato H*, Ebato K*, Saito S, Miyata N, Imamura T, Miyajima A#.

Biochem J. 2011 Nov 15;440(1):33-41.

 

A novel regulatory mechanism for Fgf18 signaling involving cysteine-rich FGF receptor (Cfr) and delta-like protein (Dlk).

Miyaoka Y, Tanaka M, Imamura T, Takada S, Miyajima A#.

Development. 2010 Jan;137(1):159-67.

 

TRB2, a mouse Tribbles ortholog, suppresses adipocyte differentiation by inhibiting AKT and C/EBPbeta.

Naiki T, Saijou E, Miyaoka Y, Sekine K, Miyajima A#.

J Biol Chem. 2007 Aug 17;282(33):24075-82.

 

Oncostatin M inhibits adipogenesis through the RAS/ERK and STAT5 signaling pathways.

Miyaoka Y, Tanaka M, Naiki T, Miyajima A#.

J Biol Chem. 2006 Dec 8;281(49):37913-20.

 *Authors contributed equally     #Corresponding author