A paper of my postdoc work was accepted at Scientific Reports. It took too long, but I am happy that it is finally done. In our previous Nature Methods paper, we employed droplet digital PCR (ddPCR) to detect homology-directed repair (HDR). In the accepted paper, we improved the method to detect both HDR and non-homologous end joining (NHEJ) at the same time. By using this method, we systematically measured HDR- and NHEJ-inducing activities of many different genome editing conditions. We found strong nuclease type-, genome locatin-, and cell type-dependencies of genome editing outcomes, which had not been fully known.
It is always tough to publish a paper, and always relieving to get it done. I thank my great coworkers and collaborators. Also, the intern who worked with me and significantly contributed to the accepted paper told me that he has got an offer from a graduate school. I am so happy with it. I was able to make the first TAE buffer and the first Ampicillin aliquots in the lab today. Therefore, today is a good day, and I hope there will be more celebrations to come.