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First iPS Cell Culture in the Lab

Lab setup is ongoing. Now I can do PCR and DNA cloning. Finally, I started culturing human iPS cells in the lab. The media, ROCK inhibitor, and Matrigel.. Everything is so expensive. Moreover, this was a critical step. If I cannot culture iPS cells here, I won't be able to run this lab at all. Therefore, I was kind of nervous checking the cells under a microscope several times a day. This was my first time I got this nervous about iPS cells since I cultured them for the first time 5 years ago at Gladstone Institutes. Fortunately, the cells are growing well. I am happy.

I will try yet another key technique, ddPCR next week.




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