Polyacrylamide Gel Electrophoresis
I tried the first polyacrylamide gel electrophoresis in the lab. The purpose was to analyze small DNA fragments, so it was a TBE gel not for Western blotting.
I did basically the same experiment several times as a postdoc. I used precast gels at that time, but I do not have that luxury now (of course not), so I made one.
I think I did just a couple of Western blots during my postdoc. As a graduate student, I did literally hundreds of Western blots. Also, I did hundreds of Northern blots as a graduate student, but have never done a single since then. It is interesting to note how different required techniques are depending on what I want to study.
Fortunately, I had no problem in making or running the gel. It worked very well.
By the way, the results were negative though.
It's alright. When I can clearly identify negative results as negative, I would say that is a success.